eIF4E (8C8) Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB3072-50, MB3072-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: P06730

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB,

All Applications: WB: 1/500-1/1000

Background: eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG1

Bioworld Molecular Weight: Calculated MW: 25 kDa; Observed MW: 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of eIF4E in MDA-MB-468, ZR751, MCF-7, T47D and CHO-K1 lysates using eIF4E antibody., Western blot analysis of eIF4E in Jurkat, Hela, MCF-7, K562, C6, 3T3, PC-12, HepG2, CHO-K1, Raji, Raw264. 7 and Ramos lysates using eIF4E antibody.

Alternative Name: EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

Immunogen: Purified recombinant human eif4e protein expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodified