POLR1A Polyclonal Antibody

Catalogue Numbers: BS60144-50, BS60144-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95602

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: RNA polymerases transcribe nuclear genes for ribosomal RNA, thus representing ribosomal biogenesis. RNA polymerase I (Pol I) is located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription machinery are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I transcription. RPA40 and RPA16 are subunits of Pol I that associate with each other at an early stage of RNA polymerase I assembly. RPA40 is essential for the function and integrity of the complex and is also an essential subunit of RNA polymerase III (Pol III). RPA40, RPA16 and RPA135 encode the three subunits of RNA polymerase I, respectively. RPA194 is the largest subunit of RNA Pol I and is not a component of Pol II and Pol III.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: POLR1A polyclonal antibody detects endogenous levels of POLR1A protein.

Extra Notes: Western blot (WB) analysis of POLR1A pAb at 1:1000 dilution
Lane1:Hela whole cell lysate(40ug)
Lane2:MCF-7 whole cell lysate(40ug)
Lane3:3T3-L1 whole cell lysate(40ug)
Lane4:Raw264.7 whole cell lysate (40ug)
Lane5:PC12 whole cell lysate (40ug)

Bioworld Molecular Weight: ~ 250 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: DNA-directed RNA polymerase I subunit RPA1; RNA polymerase I subunit A1; A190; DNA-directed RNA polymerase I largest subunit; DNA-directed RNA polymerase I subunit A; RNA polymerase I 194 kDa subunit; RPA194; POLR1A

Immunogen: A synthetic peptide corresponding to residues in Human POLR1A.

Conjugate: Unconjugated

Modification: Unmodified