p-H2BFS(K15)  Polyclonal Antibody

Catalogue Numbers: BS64415-50, BS64415-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P57053

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation,phosphorylation,sumoylation, ubiquitination and ADP-ribosylation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: H2BFS(phospho-K15) polyclonal antibody detects endogenous levels of H2BFS(phospho-K15) protein.

Extra Notes: Western blot (WB) analysis of H2BFS(phospho-K15) polyclonal antibody at 1:500 dilution
Lane1:The thymus tissue lysate of Rat(40ug)
Lane2:The spleen tissue lysate of Mouse(40ug)
Lane3:HuT78 whole cell lysate(40ug)
Lane4:SHSY5Y whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 17kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Histone H2B type F-S;Histone H2B.s;H2B/s;H2BFS;

Immunogen: Synthetic peptide, corresponding to Human H2BFS(phospho-K15) .

Conjugate: Unconjugated

Modification: Unmodified