BORA Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-53604-60, E-AB-53604-120, E-AB-53604-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: BORA

Target Synonym: Aurora borealis; bora; bora; aurora kinase A activator; BORA; C13orf34; Chromosome 13 open reading frame 34 ; FLJ22624; HsBora; Protein aurora borealis; RP11-342J4.2

Research Areas: Cancer, Cell Biology, Signal transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB, IHC, ELISA

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: Q6PGQ7

Background: Aurora related kinase-1 (ARK-1) is a centrosome-associated serine/threonine kinase that regulates centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. Bora (protein aurora borealis) is a 559 amino acid protein that activates ARK-1. Bora is localized to the nucleus until mitosis is initiated, when it then translocates to the cytoplasm. This translocation is dependent on activated Cdc2, which releases Bora to bind and activate ARK-1 in the cytoplasm. Plk (polo-like kinase) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation on its N-terminus by ARK-1. It is through this mechanism that Bora and ARK-1 control cellular mitotic entry. Downregulation of the gene encoding Bora results in multipolar spindles in mitosis, a phenomenon that is also observed when ARK-1 function is blocked.

Concentration: 1.8 mg/mL

Immunogen: Synthetic peptide of human BORA

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Purification Method: Antigen affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Calculated MW: 61 kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only