Cortactin Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB10754-50, MB10754-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q14247

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF, IP,

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background: Cortactin a cytoskeletal protein that that is involved in coordinating actin reorganization during cell movement. Localizes at the leading edge of lamellipodia during cell migration. Its amino-terminal acidic domain associates with the Arp2/3 and WASP complex at F-actin branches.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 62 kDa; Observed MW: 80-85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cortactin in K562, rat Brain, C6, 3T3, Hela lysates using Cortactin antibody., Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Cortactin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunocytochemistry analysis of Cortactin in Hela using Cortactin antibody, and DAPI

Alternative Name: CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1

Immunogen: A synthetic peptide of human Cortactin

Conjugate: Unconjugated

Modification: Unmodified